Identification and Verification of Differential Display cDNAs Using Gene-Specific Primers and Hybridization Arrays
An accurate and streamlined approach to differential display (DD) band identification and verification is described.To minimize false positives, the strategy avoids the use of impure Northern blot probes obtained from PCR-amplified DD bands.To increase throughput, the cloning of DD bands is replaced by a gene-specific primer approach, angilina whit